NGSgo®-MX11-3

Benefits

• High quality results
• Whole-gene coverage
• RUO & CE-IVD

Description

NGSgo-MX11-3 features amplification primers for eleven loci, multiplexed in three tubes. The three-tube design results in lower noise levels, better balanced alleles, and higher quality results compared to a one-tube approach. This means more reliable typing results.

For the amplification of HLA-A, -B, -C, -DRB1, -DQB1 and –DPB1, DRB3/4/5, DQA1, and DPA1. A ready-to-use PCR mastermix (GenDx-LongMix) is included in the kit.

Available as RUO and CE-marked-IVD.

Specifications

NGSgo-MX11-3 is fast and easy to use, as multiplexing means less pipetting and pooling. Pipetting is further minimized by our GenDx-LongMix enzyme mastermix, which is included in the kit. The product is compatible with the NGSgo workflow, for a library prep with minimal cleanups and quantifications.

With NGSgo-MX11-3 you get from DNA to report in only 1,5 days. Start your amplification on day 1 and have your typing report on day 2 before noon. You can run 16 samples on an Illumina micro flow cell.

HLA locus-specific amplification is performed in a thermal cycler using the primer mix, DNA, enzyme mastermix, and water. The resulting HLA locus-specific amplicons can be used for identification of HLA alleles by means of Next-generation sequencing.

Amplification coverage

To be supplied by the user:

• Cold block or ice
• Pipettes and pipette tips with hydrophobic filters
• Thermal cycler
• Microcentrifuge

For more information please Contact Us or see this product on the GENDX Website.

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