NGSgo®-AmpX v2 (incl. LongMix)

Singleplex amplification primer sets including polymerase enzyme

Benefits

  • Optimized version of AmpX
  • Single loci
  • Includes enzyme mastermix

Description

NGSgo-AmpX v2 is an optimized version of NGSgo-AmpX with a faster cycling time, colored primer pellet, and a convenient enzyme mastermix (GenDx-LongMix) included in the kit. Available for the amplification of the following HLA genes: HLA-A, -B, -C, -DRB1, -DQB1, -DPA1, -DPB1, -DQA1, -DRB3/4/5 and -G. Primers can be purchased in combined-loci kits or individually. They are compatible with all common sequencing platforms. NGSgo-AmpX v2 primer sets are available as RUO and CE-marked IVD.

 

Specifications

NGSgo-AmpX v2 features exactly the same HLA gene coverage as NGSgo-AmpX. The whole gene is amplified for the Class I HLA genes HLA-A, B and C. For class II HLA genes with extremely large introns, only the most relevant parts of the genes are amplified. For -DRB1 and -DQB1 there are also options with more extended coverage amplifying the whole (or almost the whole) gene.

NGSgo-AmpX v2 HLAGeneSuite amplification coverage

 

 

NGSgo-AmpX v2 whole gene amplification coverage

 

To be supplied by the user:

  • Cold block
  • Pipettes and pipette tips with hydrophobic filters
  • Thermal cycler
  • Microcentrifuge
  • Vortex
  • PCR tubes (thin-walled 0.2 ml PCR tubes recommended by the manufacturer of your thermal cycler)
  • Agarose gel electrophoresis system

For more information please Contact Us or see the GENDX Website.

Product Table

Product Reference Reactions # Catalog #
HLA-A

96

7370162

HLA-B

96

7370262

HLA-C

96

7370362

HLA-DRB1

96

7370662

HLA-DRB1 All Exon

96

7370622

HLA-DRB3/4/5

96

7370762

HLA-DQA1

96

7370862

HLA-DQB1

96

7370562

HLA-DQB1 Whole Gene

96

7370512

HLA-DPA1

96

7370962

HLA-DPB1

96

7370462

HLA-G

96

7371062

HLA-A, B, C, DRB1, DQB1, DPB1

96

7371462

HLAGeneSuite

96

7371662

Multiplex A, B, C, DRB1, DQB1, DPB1

96

7371464