NGSgo®-MX11-3
Multiplexed HLA amplification 11 loci
Benefits
- High quality results
- Whole-gene coverage
- Includes enzyme mastermix
Description
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NGSgo-MX11-3 features amplification primers for eleven loci, multiplexed in three tubes. The three-tube design results in lower noise levels, better balanced alleles, and higher quality results compared to a one-tube approach. This means more reliable typing results.
For the amplification of HLA-A, -B, -C, -DRB1, -DQB1 and –DPB1, DRB3/4/5, DQA1, and DPA1. A ready-to-use PCR mastermix (GenDx-LongMix) is included in the kit.
Specifications
NGSgo-MX11-3 is fast and easy to use, as multiplexing means less pipetting and pooling. Pipetting is further minimized by our GenDx-LongMix enzyme mastermix, which is included in the kit. The product is compatible with the NGSgo workflow, for a library prep with minimal cleanups and quantifications.
HLA locus-specific amplification is performed in a thermal cycler using the primer mix, DNA, enzyme mastermix, and water. The resulting HLA locus-specific amplicons can be used for identification of HLA alleles by means of Next-generation sequencing.
Amplification coverage
To be supplied by the user:
- Cold block or ice
- Pipettes and pipette tips with hydrophobic filters
- Thermal cycler
- Microcentrifuge
For more information please Contact Us or see the GENDX Website.
Product Table
Multiplex A,B,C,DRB1,DQB1,DPB1,DRB3/4/5,DQA1,DPA1 |
96 |
7371864 |