NGSgo®-MX11-3

Multiplexed HLA amplification 11 loci

Benefits

  • High quality results
  • Whole-gene coverage
  • Includes enzyme mastermix

Description

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NGSgo-MX11-3 features amplification primers for eleven loci, multiplexed in three tubes. The three-tube design results in lower noise levels, better balanced alleles, and higher quality results compared to a one-tube approach. This means more reliable typing results.

For the amplification of HLA-A, -B, -C, -DRB1, -DQB1 and –DPB1, DRB3/4/5, DQA1, and DPA1. A ready-to-use PCR mastermix (GenDx-LongMix) is included in the kit.

Specifications

NGSgo-MX11-3 is fast and easy to use, as multiplexing means less pipetting and pooling. Pipetting is further minimized by our GenDx-LongMix enzyme mastermix, which is included in the kit. The product is compatible with the NGSgo workflow, for a library prep with minimal cleanups and quantifications.

HLA locus-specific amplification is performed in a thermal cycler using the primer mix, DNA, enzyme mastermix, and water. The resulting HLA locus-specific amplicons can be used for identification of HLA alleles by means of Next-generation sequencing.

 

Amplification coverage

 

To be supplied by the user:

  • Cold block or ice
  • Pipettes and pipette tips with hydrophobic filters
  • Thermal cycler
  • Microcentrifuge

For more information please Contact Us or see the GENDX Website.

Product Table

Multiplex A,B,C,DRB1,DQB1,DPB1,DRB3/4/5,DQA1,DPA1

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