NGSgo®-AmpX MICA, MICB

Benefits

• MICA & MICB typing
• Includes enzyme mastermix
• Compatible with LibrX and IndX

Description

NGSgo-AmpX MICA, MICB includes amplification primers for whole-gene amplification of MICA and MICB. The kit also includes the GenDx-LongMix enzyme mastermix, and is compatible with NGSgo library preparation and analysis in NGSengine. Available as RUO.

Specifications

The MHC Class I chain-related genes A (MICA) and B (MICB) are located on chromosome 6, close to HLA-B. MICA and MICB genes are similar in structure to HLA Class I, but they do not bind peptides and do not engage with the T-cell receptor. Instead they engage with an activating receptor, NKG2D, expressed on the surface of NK cells and certain T cells. This interaction stimulates the cytolytic activity and cytokine production of these cells, and thereby influences immunological responses.

Amplification coverage

In solid organ transplantation, anti-MICA antibodies have been found to be a risk factor for both acute and chronic rejection. In stem cell transplantation, MICA mismatching has been associated with graft-versus-host disease, relapse, and overall mortality. Different studies have reported conflicting results though, therefore the effect of MICA donor-recipient mismatching remains controversial.  In disease association studies, certain MICA polymorphisms have shown to be associated with various diseases including ankylosing spondylitis, inflammatory bowel disease, and diabetes.

To be supplied by the user:

• Cold block or ice
• Pipettes and pipette tips with hydrophobic filters
• Thermal cycler
• Microcentrifuge
• Vortex
• PCR tubes or PCR plate (thin-walled 0.2 ml PCR tubes recommended by the manufacturer of your thermal cycler)
• Agarose gel electrophoresis system

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