NGSgo®-MX6-1

Multiplexed HLA amplification 6 loci

Benefits

  • One-Day-Workflow
  • Includes enzyme mastermix

Description

NGSgo-MX6-1 features six loci in one tube for the amplification of HLA-A, -B, -C, -DRB1, -DQB1 and –DPB1. A ready-to-use PCR mastermix (GenDx-LongMix) is included in the kit.

One-Day-Workflow: NGSgo-MX6-1 makes it possible to perform 6-loci HLA typing within 24 hours (10 samples on Illumina MiSeq Nano flow cell). This includes data analysis.

NGSgo-MX6-1 has been validated with library preparation reagents of NGSgo Workflow compatible with Illumina and is available as RUO and CE-marked IVD.

Specifications

Multiplexing significantly reduces pipetting steps and hands-on time because fewer amplification mixes are needed. Additionally, the mixes are more easily prepared because of the ready-to-use enzyme mastermix that is included in the kit. You just combine mastermix, primer mix, DNA, and water. Run on your thermal cycler for <3 hours and you are ready for the library preparation. Quantification is not required after amplification.

 

Amplification coverage

To be supplied by the user:

  • Cold block or ice
  • Pipettes and pipette tips with hydrophobic filters
  • Thermal cycler
  • Microcentrifuge
  • Vortex
  • PCR tubes or PCR plate (thin-walled 0.2 ml PCR tubes recommended by the manufacturer of your thermal cycler)
  • Agarose gel electrophoresis system

For more information please Contact Us or see the GENDX Website.

Product Table

Product Reference Reactions Catalog Number
Multiplex A, B, C, DRB1, DQB1, DPB1 96 7371464