NGSgo®-MX6-1
Multiplexed HLA amplification 6 loci
Benefits
- One-Day-Workflow
- Includes enzyme mastermix
Description
NGSgo-MX6-1 features six loci in one tube for the amplification of HLA-A, -B, -C, -DRB1, -DQB1 and –DPB1. A ready-to-use PCR mastermix (GenDx-LongMix) is included in the kit.
One-Day-Workflow: NGSgo-MX6-1 makes it possible to perform 6-loci HLA typing within 24 hours (10 samples on Illumina MiSeq Nano flow cell). This includes data analysis.
NGSgo-MX6-1 has been validated with library preparation reagents of NGSgo Workflow compatible with Illumina and is available as RUO and CE-marked IVD.
Specifications
Multiplexing significantly reduces pipetting steps and hands-on time because fewer amplification mixes are needed. Additionally, the mixes are more easily prepared because of the ready-to-use enzyme mastermix that is included in the kit. You just combine mastermix, primer mix, DNA, and water. Run on your thermal cycler for <3 hours and you are ready for the library preparation. Quantification is not required after amplification.
Amplification coverage
To be supplied by the user:
- Cold block or ice
- Pipettes and pipette tips with hydrophobic filters
- Thermal cycler
- Microcentrifuge
- Vortex
- PCR tubes or PCR plate (thin-walled 0.2 ml PCR tubes recommended by the manufacturer of your thermal cycler)
- Agarose gel electrophoresis system
For more information please Contact Us or see the GENDX Website.
Product Table
Product Reference | Reactions | Catalog Number |
Multiplex A, B, C, DRB1, DQB1, DPB1 | 96 | 7371464 |