NGSgo®-MX6-1

Benefits

• One-Day-Workflow
• Includes enzyme mastermix

Description

NGSgo-MX6-1 features six loci in one tube for the amplification of HLA-A, -B, -C, -DRB1, -DQB1 and –DPB1. A ready-to-use PCR mastermix (GenDx-LongMix) is included in the kit.

One-Day-Workflow: NGSgo-MX6-1 makes it possible to perform 6-loci HLA typing within 24 hours (10 samples on Illumina MiSeq Nano flow cell). This includes data analysis.

NGSgo-MX6-1 has been validated with library preparation reagents of NGSgo Workflow compatible with Illumina and is available as RUO and CE-marked IVD.

Specifications

Multiplexing significantly reduces pipetting steps and hands-on time because fewer amplification mixes are needed. Additionally, the mixes are more easily prepared because of the ready-to-use enzyme mastermix that is included in the kit. You just combine mastermix, primer mix, DNA, and water. Run on your thermal cycler for <3 hours and you are ready for the library preparation. Quantification is not required after amplification.

Amplification coverage

To be supplied by the user:

• Cold block or ice
• Pipettes and pipette tips with hydrophobic filters
• Thermal cycler
• Microcentrifuge
• Vortex
• PCR tubes or PCR plate (thin-walled 0.2 ml PCR tubes recommended by the manufacturer of your thermal cycler)
• Agarose gel electrophoresis system

For more information please Contact Us or see the GENDX Website.

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