NGSgo®-AmpX (Amplification primers)

Benefits

• Succesful strategy since 2005
• Single loci
• Choice of 12 different HLA genes

Description

NGSgo-AmpX primer sets are available for amplification of the following HLA genes: HLA-A, -B, -C, -DRB1, -DQB1, -DPA1, -DPB1, -DQA1, -DRB3/4/5 and -G. Primers can be purchased in combined-loci kits or individually. Compatible with all common sequencing platforms. NGSgo-AmpX primer sets are available as RUO and CE-marked IVD.

Specifications

In this singleplex approach, the whole gene is amplified for the Class I HLA genes. For class II HLA genes with extremely large introns, only the most relevant parts of the genes are amplified.

Amplification coverage

To be supplied by the user:

• GenDx or QIAGEN LongRange PCR Kit
• Pipettes and pipette tips with hydrophobic filters
• Thermal cycler
• Micro centrifuge
• Vortexer
• PCR tubes (thin-walled 0.2 ml PCR tubes recommended by the manufacturer of your thermal cycler)
• Agarose gel electrophoresis system

For more information please Contact Us or see the GENDX Website.

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