NGSgo®-AmpX (Amplification primers)

Singleplex amplification primers with proven reliability

Benefits

  • Succesful strategy since 2005
  • Single loci
  • Choice of 12 different HLA genes

Description

NGSgo-AmpX primer sets are available for amplification of the following HLA genes: HLA-A, -B, -C, -DRB1, -DQB1, -DPA1, -DPB1, -DQA1, -DRB3/4/5 and -G. Primers can be purchased in combined-loci kits or individually. Compatible with all common sequencing platforms. NGSgo-AmpX primer sets are available as RUO and CE-marked IVD.

Specifications

In this singleplex approach, the whole gene is amplified for the Class I HLA genes. For class II HLA genes with extremely large introns, only the most relevant parts of the genes are amplified.

Amplification coverage

 

To be supplied by the user:

  • GenDx or QIAGEN LongRange PCR Kit
  • Pipettes and pipette tips with hydrophobic filters
  • Thermal cycler
  • Micro centrifuge
  • Vortexer
  • PCR tubes (thin-walled 0.2 ml PCR tubes recommended by the manufacturer of your thermal cycler)
  • Agarose gel electrophoresis system

For more information please Contact Us or see the GENDX Website.

Product Table

HLA-A

24

2340102

96

2340162

384

2340142

HLA-B

24

2340202

96

2340262

384

2340242

HLA-C

24

2340302

96

2340362

384

2340342

HLA-DRB1

24

2340602

96

2340662

384

2340642

HLA-DRB3/4/5

24

2340702

96

2340762

384

2340742

HLA-DQA1

24

2340802

96

2340862

384

2340842

HLA-DQB1

24

2340502

96

2340562

384

2340542

HLA-DPA1

24

2340902

96

2340962

384

2340942

HLA-DPB1

24

2340402

96

2340462

384

2340442

HLA-A, B, C, DRB1, DQB1

24

2341102

96

2341162

384

2341142

HLA-DPA1, DPB1, DQA1, DRB3/4/5

24

2341502

96

2341562

384

2341542

HLA GeneSuite

24

2341602

96

2341662

384

2341642

HLA-E (prototype)

24

2341202

HLA-F (prototype)

24

2341702

HLA-G

24

2341002