NGSgo®-AmpX (Amplification primers)
Singleplex amplification primers with proven reliability
Benefits
- Succesful strategy since 2005
- Single loci
- Choice of 12 different HLA genes
Description
NGSgo-AmpX primer sets are available for amplification of the following HLA genes: HLA-A, -B, -C, -DRB1, -DQB1, -DPA1, -DPB1, -DQA1, -DRB3/4/5 and -G. Primers can be purchased in combined-loci kits or individually. Compatible with all common sequencing platforms. NGSgo-AmpX primer sets are available as RUO and CE-marked IVD.
Specifications
In this singleplex approach, the whole gene is amplified for the Class I HLA genes. For class II HLA genes with extremely large introns, only the most relevant parts of the genes are amplified.
Amplification coverage
To be supplied by the user:
- GenDx or QIAGEN LongRange PCR Kit
- Pipettes and pipette tips with hydrophobic filters
- Thermal cycler
- Micro centrifuge
- Vortexer
- PCR tubes (thin-walled 0.2 ml PCR tubes recommended by the manufacturer of your thermal cycler)
- Agarose gel electrophoresis system
For more information please Contact Us or see the GENDX Website.
Product Table
HLA-A |
24 |
2340102 |
|
96 |
2340162 |
||
384 |
2340142 |
||
HLA-B |
24 |
2340202 |
|
96 |
2340262 |
||
384 |
2340242 |
||
HLA-C |
24 |
2340302 |
|
96 |
2340362 |
||
384 |
2340342 |
||
HLA-DRB1 |
24 |
2340602 |
|
96 |
2340662 |
||
384 |
2340642 |
||
HLA-DRB3/4/5 |
24 |
2340702 |
|
96 |
2340762 |
||
384 |
2340742 |
||
HLA-DQA1 |
24 |
2340802 |
|
96 |
2340862 |
||
384 |
2340842 |
||
HLA-DQB1 |
24 |
2340502 |
|
96 |
2340562 |
||
384 |
2340542 |
||
HLA-DPA1 |
24 |
2340902 |
|
96 |
2340962 |
||
384 |
2340942 |
||
HLA-DPB1 |
24 |
2340402 |
|
96 |
2340462 |
||
384 |
2340442 |
||
HLA-A, B, C, DRB1, DQB1 |
24 |
2341102 |
|
96 |
2341162 |
||
384 |
2341142 |
||
HLA-DPA1, DPB1, DQA1, DRB3/4/5 |
24 |
2341502 |
|
96 |
2341562 |
||
384 |
2341542 |
||
HLA GeneSuite |
24 |
2341602 |
|
96 |
2341662 |
||
384 |
2341642 |
||
HLA-E (prototype) |
24 |
2341202 |
|
HLA-F (prototype) |
24 |
2341702 |
|
HLA-G |
24 |
2341002 |